Quantifying resolution limiting factors in subtomogram averaged cryo-electron tomography using simulations.

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第一作者： Lenard M,Voortman

第一单位： Quantitative Imaging Group, Department of Imaging Physics, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.

作者： Lenard M,Voortman ^[1] ; Miloš,Vulović ^[2] ; Massimiliano,Maletta ^[3] ; Andreas,Voigt ^[4] ; Erik M,Franken ^[4] ; Angelita,Simonetti ^[5] ; Peter J,Peters ^[6] ; Lucas J,van Vliet ^[1] ; Bernd,Rieger ^[7]

作者单位： Quantitative Imaging Group, Department of Imaging Physics, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands. ^[1] Quantitative Imaging Group, Department of Imaging Physics, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands; Institute of Nanoscopy, Faculty of Health, Medicine and Life Sciences, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. ^[2] NKI Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Plesmanlaan 121 B6, 1066 CX Amsterdam, The Netherlands. ^[3] FEI Company, Achtseweg Noord 5, 5651 GG Eindhoven, The Netherlands. ^[4] Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 67084 Strasbourg Cedex, France. ^[5] Institute of Nanoscopy, Faculty of Health, Medicine and Life Sciences, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands; NKI Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Plesmanlaan 121 B6, 1066 CX Amsterdam, The Netherlands. ^[6] Quantitative Imaging Group, Department of Imaging Physics, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands. Electronic address: b.rieger@tudelft.nl. ^[7]

关键词 Acquisition protocol Cryo-EM Ribosome Subtomogram averaging TEM image simulation Tilted CTF correction Tomography

主题词冷冻电子显微镜检查(Cryoelectron Microscopy);电子显微镜体层摄影术(Electron Microscope Tomography);图像处理, 计算机辅助(Image Processing, Computer-Assisted);大分子物质(Macromolecular Substances);核糖体(Ribosomes)

DOI 10.1016/j.jsb.2014.06.007

PMID 24998892

发布时间 2017-09-22

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