Comparison of two FFPE preparation methods using label-free shotgun proteomics: Application to tissues of diverticulitis patients.

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作者： Florence,Quesada-Calvo ^[1] ; Virginie,Bertrand ^[2] ; Rémi,Longuespée ^[3] ; Agnès,Delga ^[4] ; Gabriel,Mazzucchelli ^[3] ; Nicolas,Smargiasso ^[3] ; Dominique,Baiwir ^[5] ; Philippe,Delvenne ^[6] ; Michel,Malaise ^[7] ; Marie-Claire,De Pauw-Gillet ^[2] ; Edwin,De Pauw ^[3] ; Edouard,Louis ^[1] ; Marie-Alice,Meuwis ^[8]

作者单位： Gastroenterology Department, GIGA-R, Liège University Hospital CHU, ULg, Liège, Belgium. ^[1] Department of Preclinical and Biomedical Sciences, Mammalian Cell Culture Laboratory, GIGA-R, ULg, Liège, Belgium. ^[2] Chemistry Department, Laboratory of Mass Spectrometry, GIGA-R, CART, ULg, Liège, Belgium. ^[3] Biobanque universitaire, Liège University Hospital CHU, Liège, Belgium. ^[4] GIGA Proteomic Facility, ULg, Liège, Belgium. ^[5] Department of Preclinical and Biomedical Sciences, Laboratory of Cytology and Histology, GIGA-R, ULg, Liège, Belgium. ^[6] Department of Clinical Sciences, Department of Rheumatology, Liège University Hospital CHU, Liège, Belgium. ^[7] Gastroenterology Department, GIGA-R, Liège University Hospital CHU, ULg, Liège, Belgium. Electronic address: marie-alice.meuwis@ulg.ac.be. ^[8]

关键词 Antigen retrieval Data independent FFPE Filter aided sample preparation (FASP)Label-free Shotgun proteomics