Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382.

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作者： Jelle B,Bultema ^[1] ; Bas J H,Kuipers ^[2] ; Lubbert,Dijkhuizen ^[1]

作者单位： Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. ^[1] FrieslandCampina Research, Stationsplein 4, 3818 LE Amersfoort, The Netherlands. ^[2]

关键词 Bacillus circulans Catalytic residue DNA, deoxyribonucleic acid GH2, glycoside hydrolase family 2 GOS, galacto-oligosaccharides Galacto-oligosaccharides Mutagenesis PCR, polymerase chain reaction SD, standard deviation Tm, melting temperatures Transglycosylation activity o-NPG, ortho-nitrophenyl-β-galactoside β-Galactosidase

DOI 10.1016/j.fob.2014.11.002

PMID 25473598

发布时间 2018-11-13

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FEBS open bio

2014年4卷

1015-20页

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Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382.

FEBS open bio

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Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382.

FEBS open bio

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FEBS open bio

相关期刊

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