Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice.
作者:
关键词
Bsd, blasticidinDAPI, 4′, 6-Diamidino-2-PhenylindoleEGFP, enhanced green fluorescent proteinES cells, embryonic stem cellsFISH, fluorescent in situ hybridizationHAC, human artificial chromosomeHAT, hypoxanthine-aminopterin-thymidineMMCT, microcell mediated chromosome transferPBS, phosphate buffered salinePFA, paraformaldehydedpc, days post coitumembryonic stem cellsgene therapyhuman artificial chromosomesiPS cells, induced pluripotent stem cells
主题词
动物(Animals);胚泡(Blastocyst);CHO细胞(CHO Cells);细胞分化(Cell Differentiation);染色体, 人工, 人(Chromosomes, Artificial, Human);仓鼠亚科(Cricetinae);仓鼠属(Cricetulus);女(雌)性(Female);基因转移技术(Gene Transfer Techniques);人类(Humans);免疫组织化学(Immunohistochemistry);原位杂交, 荧光(In Situ Hybridization, Fluorescence);小鼠(Mice);小鼠, 近交C57BL(Mice, Inbred C57BL);畸胎瘤(Teratoma)
DOI
10.1080/15384101.2015.1014151
PMID
25695642
发布时间
2020-09-30
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Cell cycle (Georgetown, Tex.)
1268-73页
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