换一批
换一批A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications.
作者：
关键词
3-MA, 3-methyladenineCyto-IDFBS, fetal bovine serumGFP, green fluorescent proteinLAMP1, lysosomal-associated membrane protein 1MAP1LC3B/LC3B, microtubule-associated protein 1 light chain 3 betaMAP3K6, mitogen-activated protein kinase kinase kinase 6MDC, monodansylcadaverineMTOR, mechanistic target of rapamycinNS, nonsilencingRAB5A, member RAS oncogene familyRNA interference screenRNAi, RNA interferenceSQSTM1, sequestosome 1WNK2, WNK lysine deficient protein kinase 2autophagyautophagy fluxautophagy responsemRFP, monomeric red fluorescent proteinshRNA, short-hairpin RNAsmall molecule screenspectrophotometric assay
主题词
动物(Animals);自噬(Autophagy);细胞存活(Cell Survival);氯喹(Chloroquine);更生霉素(Dactinomycin);内颗粒(Endosomes);荧光染料(Fluorescent Dyes);基因表达调控, 白血病(Gene Expression Regulation, Leukemic);HEK293细胞(HEK293 Cells);人类(Humans);K562细胞(K562 Cells);白血病(Leukemia);溶酶体(Lysosomes);MAP激酶激酶激酶类(MAP Kinase Kinase Kinases);小鼠(Mice);小鼠, 近交C57BL(Mice, Inbred C57BL);小鼠, SCID(Mice, SCID);显微镜检查, 荧光(Microscopy, Fluorescence);肿瘤移植(Neoplasm Transplantation);RNA干扰(RNA Interference);光谱法, 荧光(Spectrometry, Fluorescence)
DOI
10.1080/15548627.2015.1017181
PMID
25714620
发布时间
2021-12-03
- 浏览129
Autophagy
560-72页
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文
