Evaluating kinase ATP uptake and tyrosine phosphorylation using multiplexed quantification of chemically labeled and post-translationally modified peptides.

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第一作者： Bin,Fang

第一单位： Proteomics, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States.

作者： Bin,Fang ^[1] ; Melissa A,Hoffman ^[2] ; Abu-Sayeef,Mirza ^[2] ; Katie M,Mishall ^[2] ; Jiannong,Li ^[3] ; Scott M,Peterman ^[4] ; Keiran S M,Smalley ^[5] ; Kenneth H,Shain ^[3] ; Paul M,Weinberger ^[6] ; Jie,Wu ^[3] ; Uwe,Rix ^[3] ; Eric B,Haura ^[3] ; John M,Koomen ^[7]

作者单位： Proteomics, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States. ^[1] Molecular Oncology, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States. ^[2] Chemical Biology and Molecular Medicine, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States. ^[3] Biomarker Research Initiative in Mass Spectrometry, ThermoFisher Scientific, Cambridge, MA, United States. ^[4] Cancer Biology and Evolution, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States. ^[5] Department of Otolaryngology, Center for Biotechnology and Genomic Medicine, Georgia Regents Cancer Center, Georgia Regents University, United States. ^[6] Molecular Oncology, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States; Chemical Biology and Molecular Medicine, Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, United States. Electronic address: john.koomen@moffitt.org. ^[7]

关键词 Activity-based protein profiling Cancer Kinase signaling Liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM)Phosphotyrosine profiling Spectral library