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首页> eLife> In vitro generation of human pluripotent stem cell derived lung organoids.

In vitro generation of human pluripotent stem cell derived lung organoids.

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作者： Briana R,Dye ^[1] ; David R,Hill ^[2] ; Michael A H,Ferguson ^[2] ; Yu-Hwai,Tsai ^[2] ; Melinda S,Nagy ^[2] ; Rachel,Dyal ^[2] ; James M,Wells ^[3] ; Christopher N,Mayhew ^[3] ; Roy,Nattiv ^[4] ; Ophir D,Klein ^[4] ; Eric S,White ^[2] ; Gail H,Deutsch ^[5] ; Jason R,Spence ^[1]

作者单位： Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, United States. ^[1] Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United States. ^[2] Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, United States. ^[3] Institute for Human Genetics, Department of Pediatrics, University of California, San Francisco, San Francisco, United States. ^[4] Department of Laboratories, Seattle Children's Hospital and University of Washington, Seattle, United States. ^[5]

关键词 developmental biology endoderm foregut human lung organoids pluripotent stem cells spheroid stem cells

主题词细胞培养技术(Cell Culture Techniques);细胞分化(Cell Differentiation);细胞系(Cell Line);细胞, 培养的(Cells, Cultured);胚胎干细胞(Embryonic Stem Cells);内胚层(Endoderm);基因表达谱(Gene Expression Profiling);人类(Humans);诱导多能干细胞(Induced Pluripotent Stem Cells);肺(Lung);显微镜检查, 共焦(Microscopy, Confocal);显微镜检查, 电子, 透射(Microscopy, Electron, Transmission);器官发生(Organogenesis);类器官(Organoids);多潜能干细胞(Pluripotent Stem Cells);结果可重复性(Reproducibility of Results);逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction);球形体, 细胞(Spheroids, Cellular);组织工程(Tissue Engineering)

DOI 10.7554/eLife.05098

PMID 25803487

发布时间 2022-03-18

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