Multiparameter optimization method and enhanced production of secreted recombinant single-chain variable fragment against the HIV-1 P17 protein from Escherichia coli by fed-batch fermentation.

导出 LinkOut

翻译打印收藏纠错

第一作者： Porntip,Paopang

第一单位： a Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences , Chiang Mai University , Chiang Mai , Thailand.

作者： Porntip,Paopang ^[1] ; Watchara,Kasinrerk ^[2] ; Chatchai,Tayapiwatana ^[2] ; Phisit,Seesuriyachan ^[3] ; Bordin,Butr-Indr ^[1]

作者单位： a Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences , Chiang Mai University , Chiang Mai , Thailand. ^[1] b Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences , Chiang Mai University , Chiang Mai , Thailand.;c Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences , Chiang Mai University , Chiang Mai , Thailand. ^[2] d Department of Biotechnology, Faculty of Agro-Industry , Chiang Mai University , Chiang Mai , Thailand. ^[3]

关键词 Fed-batch cultivation Plackett–Burman design multiparameter optimization scFv anti-p17 secreted recombinant protein sequential simplex optimization