Rapid method to express and purify human membrane protein using the Xenopus oocyte system for functional and low-resolution structural analysis.
第一作者:
Benjamin,Clémençon
第一单位:
Institute of Biochemistry and Molecular Medicine (IBMM), University of Bern, Bern, Switzerland; Swiss National Centre of Competence in Research (NCCR) TransCure, University of Bern, Bern, Switzerland. Electronic address: benjamin.clemencon@ibmm.unibe.ch.
作者:
医学主题词
动物(Animals);克隆, 分子(Cloning, Molecular);女(雌)性(Female);基因表达(Gene Expression);人类(Humans);膜蛋白质类(Membrane Proteins);显微镜检查, 电子, 透射(Microscopy, Electron, Transmission);卵母细胞(Oocytes);RNA(RNA);光滑爪蟾(Xenopus laevis)
DOI
10.1016/bs.mie.2014.12.011
PMID
25857785
发布时间
2015-04-10
- 浏览12
Methods in enzymology
241-65页
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