Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules.

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作者： C F,Martins ^[1] ; A E D Feliciano,Silva ^[2] ; M N,Dode ^[3] ; R,Rumpf ^[4] ; H C B,Cumpa ^[5] ; C G,Silva ^[6] ; I,Pivato ^[7]

作者单位： Laboratory of Reproduction, Center for Technology Transference of Zebu Dairy Cows-CTZL, Embrapa Cerrados, DF 180, Km 18, Brasília, Federal District, Brazil. Electronic address: carlos.martins@embrapa.br. ^[1] Embrapa Genetic Resources and Biotechnology, Brasília, Federal District, Brazil. Electronic address: emidiofd@yahoo.com. ^[2] Embrapa Genetic Resources and Biotechnology, Brasília, Federal District, Brazil. Electronic address: margot.dode@embrapa.br. ^[3] Embrapa Genetic Resources and Biotechnology, Brasília, Federal District, Brazil. Electronic address: rodolfo.rumpf@embrapa.br. ^[4] Laboratory of Reproduction, Center for Technology Transference of Zebu Dairy Cows-CTZL, Embrapa Cerrados, DF 180, Km 18, Brasília, Federal District, Brazil. Electronic address: heidi.cumpa@embrapa.br. ^[5] Laboratory of Reproduction, Center for Technology Transference of Zebu Dairy Cows-CTZL, Embrapa Cerrados, DF 180, Km 18, Brasília, Federal District, Brazil; University of Brasília, Brasília, Federal District, Brazil. Electronic address: carolgonzaless@gmail.com. ^[6] University of Brasília, Brasília, Federal District, Brazil. Electronic address: pivato@unb.br. ^[7]

关键词 Assisted reproduction Cryopreservation ICSI Male germplasm Spermatids