Development of three triplex real-time reverse transcription PCR assays for the qualitative molecular typing of the nine serotypes of African horse sickness virus.

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作者： Camilla T,Weyer ^[1] ; Christopher,Joone ^[2] ; Carina W,Lourens ^[2] ; Mpho S,Monyai ^[2] ; Otto,Koekemoer ^[3] ; John D,Grewar ^[4] ; Antoinette,van Schalkwyk ^[5] ; Phelix O A,Majiwa ^[3] ; N James,MacLachlan ^[6] ; Alan J,Guthrie ^[2]

作者单位： Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. Electronic address: equineresearchcentre@gmail.com. ^[1] Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. ^[2] Agricultural Research Council, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. ^[3] Epidemiology, Veterinary Services (Animal Health) Western Cape Department of Agriculture, Private Bag X1, Elsenburg 7607, South Africa. ^[4] Agricultural Research Council, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa. ^[5] Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. ^[6]

关键词 AHS Equine Field diagnosis RT-qPCR Serotype

主题词动物(Animals);马(Horses);分子分类(Molecular Typing);RNA, 病毒(RNA, Viral);逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction);敏感性与特异性(Sensitivity and Specificity)

DOI 10.1016/j.jviromet.2015.07.015

PMID 26232526

发布时间 2019-12-10

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Journal of virological methods

2015年223卷

69-74页

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Development of three triplex real-time reverse transcription PCR assays for the qualitative molecular typing of the nine serotypes of African horse sickness virus.

Journal of virological methods

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Journal of virological methods

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Development of three triplex real-time reverse transcription PCR assays for the qualitative molecular typing of the nine serotypes of African horse sickness virus.

Journal of virological methods

相似文献

Journal of virological methods

相关期刊

检索历史清除