Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system.

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第一作者： Xiaolong,Wang

第一单位： College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

作者： Xiaolong,Wang ^[1] ; Honghao,Yu ^[2] ; Anmin,Lei ^[3] ; Jiankui,Zhou ^[4] ; Wenxian,Zeng ^[5] ; Haijing,Zhu ^[6] ; Zhiming,Dong ^[1] ; Yiyuan,Niu ^[2] ; Bingbo,Shi ^[3] ; Bei,Cai ^[6] ; Jinwang,Liu ^[1] ; Shuai,Huang ^[1] ; Hailong,Yan ^[1] ; Xiaoe,Zhao ^[2] ; Guangxian,Zhou ^[3] ; Xiaoling,He ^[2] ; Xiaoxu,Chen ^[3] ; Yuxin,Yang ^[1] ; Yu,Jiang ^[2] ; Lei,Shi ^[3] ; Xiue,Tian ^[4] ; Yongjun,Wang ^[1] ; Baohua,Ma ^[1] ; Xingxu,Huang ^[1] ; Lei,Qu ^[1] ; Yulin,Chen ^[1]

作者单位： College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. ^[1] Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, China. ^[2] Life Science Research Center, Yulin University, Yulin 719000, China. ^[3] College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China. ^[4] MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China. ^[5] School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. ^[6]

主题词动物(Animals);动物, 基因修饰(Animals, Genetically Modified);碱基序列(Base Sequence);成纤维细胞生长因子5(Fibroblast Growth Factor 5);成纤维细胞(Fibroblasts);基因打靶(Gene Targeting);山羊(Goats);微量注射(Microinjections);分子序列数据(Molecular Sequence Data);肌肉生长抑制素(Myostatin);表型(Phenotype);RNA编辑(RNA Editing);序列比对(Sequence Alignment);合子(Zygote)

DOI 10.1038/srep13878

PMID 26354037

发布时间 2024-01-04

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Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system.

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Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system.

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