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首页> PloS one> Directed Evolution and Resolution Mechanism of 1, 3-Propanediol Oxidoreductase from Klebsiella pneumoniae toward Higher Activity by Error-Prone PCR and Bioinformatics.

Directed Evolution and Resolution Mechanism of 1, 3-Propanediol Oxidoreductase from Klebsiella pneumoniae toward Higher Activity by Error-Prone PCR and Bioinformatics.

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作者： Wei,Jiang ^[1] ; Yuan,Zhuang ^[2] ; Shizhen,Wang ^[1] ; Baishan,Fang ^[3]

作者单位： Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian, 361005, China. ^[1] The Key Laboratory for Industrial Biotechnology of Fujian Higher Education, Hua Qiao University, Xiamen, Fujian, China. ^[2] Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian, 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian, 361005, China. ^[3]

主题词醇脱氢酶(Alcohol Dehydrogenase);氨基酸取代(Amino Acid Substitution);细菌蛋白质类(Bacterial Proteins);计算生物学(Computational Biology);定向分子进化(Directed Molecular Evolution);肺炎克雷伯菌(Klebsiella pneumoniae);突变, 误义(Mutation, Missense);NAD(NAD);聚合酶链反应(Polymerase Chain Reaction);蛋白质结合(Protein Binding)

DOI 10.1371/journal.pone.0141837

PMID 26528716

发布时间 2018-11-13

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PloS one

PloS one

2015年10卷11期

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