DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System.

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作者： Michiko,Kimoto ^[1] ; Ken-ichiro,Matsunaga ^[2] ; Ichiro,Hirao ^[3]

作者单位： Center for Life Science Technologies (CLST), RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. ^[1] TagCyx Biotechnologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. ^[2] PRESTO, JST, 4-1-8 Honcho, Kawaguchi-shi, Saitama, 332-0012, Japan. ^[3] Center for Life Science Technologies (CLST), RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. ihirao@riken.go.jp. ^[4] TagCyx Biotechnologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. ihirao@riken.go.jp. ^[5]

关键词 Aptamer Genetic alphabet expansion In vitro selection PCR SELEX Unnatural base pair

主题词适体, 核苷(Aptamers, Nucleotide);碱基配对(Base Pairing);基因文库(Gene Library);聚合酶链反应(Polymerase Chain Reaction);SELEX适体技术(SELEX Aptamer Technique)

DOI 10.1007/978-1-4939-3197-2_4

PMID 26552815

发布时间 2015-11-10

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