In vivo, large-scale preparation of uniformly (15)N- and site-specifically (13)C-labeled homogeneous, recombinant RNA for NMR studies.

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作者： My T,Le ^[1] ; Rachel E,Brown ^[2] ; Anne E,Simon ^[2] ; T Kwaku,Dayie ^[3]

作者单位： Department of Chemistry and Biochemistry,Center for Biomolecular Structure and Organization, University of Maryland, College Park, Maryland, USA. ^[1] Department of Chemistry and Biochemistry, Department of Cellular Biology and Molecular Genetics, Center for Biomolecular Structure and Organization, University of Maryland, College Park, Maryland, USA. ^[2] Department of Chemistry and Biochemistry,Center for Biomolecular Structure and Organization, University of Maryland, College Park, Maryland, USA. Electronic address: dayie@umd.edu. ^[3]

关键词 (15)N, (13)C isotopes Escherichia coli HNCO Experiment Nuclear magnetic resonance spectroscopy Ribonucleic acid Selective biomass method Site-specific labeling tRNA-scaffold

主题词碳同位素(Carbon Isotopes);磁共振波谱学(Magnetic Resonance Spectroscopy);氮同位素(Nitrogen Isotopes);RNA(RNA);重组, 遗传(Recombination, Genetic)

DOI 10.1016/bs.mie.2015.07.020

PMID 26577743

发布时间 2015-11-20

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