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Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus.

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第一作者： Zarah,Forsberg

第一单位： From the Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences, 1432 Ås, Norway.

作者： Zarah,Forsberg ^[1] ; Cassandra E,Nelson ^[2] ; Bjørn,Dalhus ^[3] ; Sophanit,Mekasha ^[1] ; Jennifer S M,Loose ^[1] ; Lucy I,Crouch ^[4] ; Åsmund K,Røhr ^[1] ; Jeffrey G,Gardner ^[2] ; Vincent G H,Eijsink ^[1] ; Gustav,Vaaje-Kolstad ^[5]

作者单位： From the Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences, 1432 Ås, Norway. ^[1] the Department of Biological Sciences, University of Maryland at Baltimore County, Baltimore, Maryland 21250. ^[2] the Department of Medical Biochemistry, Institute for Clinical Medicine, University of Oslo, P. O. Box 4950, Nydalen, N-0424 Oslo, Norway, the Department of Microbiology, Clinic for Diagnostics and Intervention, Oslo University Hospital, Rikshospitalet, P. O. Box 4950, Nydalen, N-0424 Oslo, Norway, and. ^[3] the Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. ^[4] From the Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences, 1432 Ås, Norway, gustav.vaaje-kolstad@nmbu.no. ^[5]

关键词 Cellvibrio japonicus carbohydrate-binding module (CBM)cellulose chitin chitinase electron paramagnetic resonance (EPR)gene knockout lytic polysaccharide monooxygenase (LPMO)x-ray crystallography

医学主题词纤维弧菌属(Cellvibrio);壳多糖(Chitin);结晶学, X线(Crystallography, X-Ray);混合功能氧化酶类(Mixed Function Oxygenases);蛋白质结构, 三级(Protein Structure, Tertiary)

DOI 10.1074/jbc.M115.700161

PMID 26858252

发布时间 2022-04-08

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