Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design.

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作者： Justin D,Smith ^[1] ; Sundari,Suresh ^[2] ; Ulrich,Schlecht ^[1] ; Manhong,Wu ^[1] ; Omar,Wagih ^[3] ; Gary,Peltz ^[4] ; Ronald W,Davis ^[3] ; Lars M,Steinmetz ^[1] ; Leopold,Parts ^[1] ; Robert P,St Onge ^[2]

作者单位： Stanford Genome Technology Center, Department of Biochemistry, Stanford University, 3165 Porter Drive, Palo Alto, CA, 94304, USA. ^[1] Department of Genetics, Stanford University School of Medicine, Stanford, California, USA. ^[2] Department of Anesthesia, Stanford University School of Medicine, Stanford University, Stanford, California, 94305, USA. ^[3] European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Genome Campus, Hinxton, CB101SD, UK. ^[4] European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117, Heidelberg, Germany. ^[5] Department of Genetics, Stanford University School of Medicine, Stanford, California, USA. leopold.parts@sanger.ac.uk. ^[6] European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117, Heidelberg, Germany. leopold.parts@sanger.ac.uk. ^[7] Current address: Wellcome Trust Sanger Institute, Hinxton, CB101SA, UK. leopold.parts@sanger.ac.uk. ^[8] Stanford Genome Technology Center, Department of Biochemistry, Stanford University, 3165 Porter Drive, Palo Alto, CA, 94304, USA. bstonge@stanford.edu. ^[9]

主题词碱基序列(Base Sequence);染色质(Chromatin);基因组, 真菌(Genome, Fungal);人类(Humans);混合功能氧化酶类(Mixed Function Oxygenases);核小体(Nucleosomes);酿酒酵母菌(Saccharomyces cerevisiae);酿酒酵母蛋白质类(Saccharomyces cerevisiae Proteins);转录启动子(Transcription Initiation Site)

DOI 10.1186/s13059-016-0900-9

PMID 26956608

发布时间 2024-01-04

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Genome biology

2016年17卷

45页

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Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design.

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Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design.

Genome biology

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Genome biology

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