Development of a rapid, sensitive TaqMan real-time RT-PCR assay for the detection of Rose rosette virus using multiple gene targets.

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作者： Binoy,Babu ^[1] ; Ayyamperumal,Jeyaprakash ^[2] ; Debra,Jones ^[2] ; Timothy S,Schubert ^[2] ; Carlye,Baker ^[2] ; Brian K,Washburn ^[3] ; Steven H,Miller ^[3] ; Kristina,Poduch ^[3] ; Gary W,Knox ^[4] ; Francisco M,Ochoa-Corona ^[5] ; Mathews L,Paret ^[6]

作者单位： North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Quincy, FL 32351, United States. Electronic address: binoybabu@ufl.edu. ^[1] Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL 32608, United States. ^[2] Department of Biological Science, Florida State University, Tallahassee, FL 32306, United States. ^[3] North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Quincy, FL 32351, United States. ^[4] Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, United States. ^[5] North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Quincy, FL 32351, United States. Electronic address: paret@ufl.edu. ^[6]

关键词 Diagnostics Emaravirus Rose rosette virus TaqMan RT-qPCR