Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease.

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第一作者： Yanke,Chen

第一单位： Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.

作者： Yanke,Chen ^[1] ; Qichang,Li ^[2] ; Jun,Yang ^[3] ; Hao,Xie ^[4]

作者单位： Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China. ^[1] School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China. ^[2] Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China. yangjun@wipm.ac.cn. ^[3] School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China. h.xie@whut.edu.cn. ^[4]

关键词 Expression and purification Integral membrane protein Maltose binding protein Steric occlusions TEV protease Tag removal

主题词枯草芽孢杆菌(Bacillus subtilis);内肽酶类(Endopeptidases);麦芽糖结合蛋白质类(Maltose-Binding Proteins);膜蛋白质类(Membrane Proteins);重组融合蛋白质类(Recombinant Fusion Proteins)

DOI 10.1007/s12010-016-2260-z

PMID 27696139

发布时间 2017-03-02

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