Kinetic Analysis and Probing with Substrate Analogues of the Reaction Pathway of the Nitrile Reductase QueF from Escherichia coli.

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第一作者： Jihye,Jung

第一单位： From the Austrian Centre of Industrial Biotechnology, Petersgasse 14.;the Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWIGraz, Petersgasse 12/1, and.

作者： Jihye,Jung ^[1] ; Tibor,Czabany ^[2] ; Birgit,Wilding ^[3] ; Norbert,Klempier ^[4] ; Bernd,Nidetzky ^[5]

作者单位： From the Austrian Centre of Industrial Biotechnology, Petersgasse 14.;the Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWIGraz, Petersgasse 12/1, and. ^[1] the Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWIGraz, Petersgasse 12/1, and. ^[2] From the Austrian Centre of Industrial Biotechnology, Petersgasse 14.;the Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010 Graz, Austria. ^[3] the Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010 Graz, Austria. ^[4] From the Austrian Centre of Industrial Biotechnology, Petersgasse 14, bernd.nidetzky@tugraz.at.;the Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWIGraz, Petersgasse 12/1, and. ^[5]

关键词 NADPH catalytic reaction pathway enzyme catalysis enzyme mechanism isothermal titration calorimetry (ITC)isotope effect nitrile reductase pre-steady-state kinetics reductase thermodynamics