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Assessing characteristics of RNA amplification methods for single cell RNA sequencing.

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第一作者： Hannah R,Dueck

第一单位： Department of Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

作者： Hannah R,Dueck ^[1] ; Rizi,Ai ^[2] ; Adrian,Camarena ^[3] ; Bo,Ding ^[2] ; Reymundo,Dominguez ^[4] ; Oleg V,Evgrafov ^[3] ; Jian-Bing,Fan ^[5] ; Stephen A,Fisher ^[6] ; Jennifer S,Herstein ^[3] ; Tae Kyung,Kim ^[7] ; Jae Mun Hugo,Kim ^[3] ; Ming-Yi,Lin ^[4] ; Rui,Liu ^[8] ; William J,Mack ^[9] ; Sean,McGroty ^[6] ; Joseph D,Nguyen ^[3] ; Neeraj,Salathia ^[5] ; Jamie,Shallcross ^[6] ; Tade,Souaiaia ^[3] ; Jennifer M,Spaethling ^[10] ; Christopher P,Walker ^[3] ; Jinhui,Wang ^[10] ; Kai,Wang ^[3] ; Wei,Wang ^[2] ; Andre,Wildberg ^[2] ; Lina,Zheng ^[2] ; Robert H,Chow ^[4] ; James,Eberwine ^[10] ; James A,Knowles ^[3] ; Kun,Zhang ^[8] ; Junhyong,Kim ^[11]

作者单位： Department of Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. ^[1] Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA. ^[2] Department of Psychiatry & The Behavioral Sciences, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. ^[3] Department of Physiology & Biophysics, Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA, USA. ^[4] Illumina, Inc., San Diego, CA, USA. ^[5] Department of Biology, University of Pennsylvania, 415 S. University Ave, Philadelphia, PA, 19104, USA. ^[6] Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.;Present address: Allen Institute for Brain Science, Seattle, WA, USA. ^[7] Department of Bioengineering, University of California at San Diego, La Jolla, CA, USA. ^[8] Department of Neurological Surgery, Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA, USA. ^[9] Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. ^[10] Department of Biology, University of Pennsylvania, 415 S. University Ave, Philadelphia, PA, 19104, USA. Junhyong@upenn.edu. ^[11]

关键词 Bioinformatics Biotechnology Genomics Single-cell RNA-sequencing

医学主题词高通量核苷酸序列分析(High-Throughput Nucleotide Sequencing);核酸扩增技术(Nucleic Acid Amplification Techniques);RNA(RNA);结果可重复性(Reproducibility of Results);敏感性与特异性(Sensitivity and Specificity);序列分析, RNA(Sequence Analysis, RNA);单细胞分析(Single-Cell Analysis)

DOI 10.1186/s12864-016-3300-3

PMID 27881084

发布时间 2024-03-26

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