Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media.

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作者： Saara,Sillanpää ^[1] ; Lenka,Kramna ^[2] ; Sami,Oikarinen ^[3] ; Markku,Sipilä ^[1] ; Markus,Rautiainen ^[1] ; Janne,Aittoniemi ^[4] ; Jussi,Laranne ^[1] ; Heikki,Hyöty ^[5] ; Ondrej,Cinek ^[2]

作者单位： Department of Otorhinolaryngology and Head and Neck Surgery, Tampere University Hospital and School of Medicine, University of Tampere, Tampere, Finland. ^[1] Department of Pediatrics, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, Prague, Czech Republic. ^[2] Department of Virology, School of Medicine, University of Tampere, Tampere, Finland. ^[3] Fimlab Laboratories, Tampere, Finland. ^[4] Department of Virology, School of Medicine, University of Tampere, Tampere, Finland; Fimlab Laboratories, Tampere, Finland. ^[5]

关键词 16S profiling acute otitis media bacteriome profiling mass sequencing next-generation sequencing

DOI 10.1128/mSphere.00006-17

PMID 28357413

发布时间 2022-03-18

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Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media.

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Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media.

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