Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples.

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第一作者： Joshua,Quick

第一单位： Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, UK.

作者： Joshua,Quick ^[1] ; Nathan D,Grubaugh ^[2] ; Steven T,Pullan ^[3] ; Ingra M,Claro ^[4] ; Andrew D,Smith ^[1] ; Karthik,Gangavarapu ^[2] ; Glenn,Oliveira ^[5] ; Refugio,Robles-Sikisaka ^[2] ; Thomas F,Rogers ^[2] ; Nathan A,Beutler ^[6] ; Dennis R,Burton ^[2] ; Lia Laura,Lewis-Ximenez ^[2] ; Jaqueline Goes,de Jesus ^[7] ; Marta,Giovanetti ^[8] ; Sarah C,Hill ^[8] ; Allison,Black ^[9] ; Trevor,Bedford ^[10] ; Miles W,Carroll ^[11] ; Marcio,Nunes ^[12] ; Luiz Carlos,Alcantara ^[11] ; Ester C,Sabino ^[3] ; Sally A,Baylis ^[13] ; Nuno R,Faria ^[14] ; Matthew,Loose ^[8] ; Jared T,Simpson ^[4] ; Oliver G,Pybus ^[15] ; Kristian G,Andersen ^[10] ; Nicholas J,Loman ^[16]

作者单位： Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, UK. ^[1] The Scripps Research Institute, La Jolla, California, USA. ^[2] Public Health England, National Infection Service, Porton Down, Salisbury, UK. ^[3] Department of Infectious Disease and Institute of Tropical Medicine, University of Saõ Paulo, Saõ Paulo, Brazil. ^[4] Scripps Translational Science Institute, La Jolla, California, USA. ^[5] Massachusetts General Hospital, Boston, Massachusetts, USA. ^[6] Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil. ^[7] Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil. ^[8] University of Rome, Tor Vergata, Italy. ^[9] Department of Zoology, University of Oxford, Oxford, UK. ^[10] Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. ^[11] Department of Epidemiology, University of Washington, Seattle, Washington, USA. ^[12] University of Southampton, South General Hospital, Southampton, UK. ^[13] Instituto Evandro Chagas, Belem, Brazil. ^[14] Paul-Ehrlich-Institut, Langen, Germany. ^[15] DeepSeq, School of Life Sciences, University of Nottingham, Nottingham, UK. ^[16] OICR, Toronto, Canada. ^[17]

主题词 DNA病毒(DNA Viruses);基因组, 病毒(Genome, Viral);分子流行病学(Molecular Epidemiology);RNA病毒(RNA Viruses);序列分析, DNA(Sequence Analysis, DNA)

DOI 10.1038/nprot.2017.066

PMID 28538739

发布时间 2024-06-20

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