Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes.

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作者： Ditte Hededam,Welner ^[1] ; David,Shin ^[2] ; Giovani P,Tomaleri ^[1] ; Andy M,DeGiovanni ^[2] ; Alex Yi-Lin,Tsai ^[1] ; Huu M,Tran ^[2] ; Sara Fasmer,Hansen ^[1] ; Derek T,Green ^[2] ; Henrik V,Scheller ^[1] ; Paul D,Adams ^[3]

作者单位： Joint BioEnergy Institute, Emeryville, California, United States of America. ^[1] Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America. ^[2] Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America. ^[3] Biological and Engineering Sciences Center, Sandia National Laboratories, Livermore, California, United States of America. ^[4]

主题词细胞壁(Cell Wall);酶激活(Enzyme Activation);基因表达(Gene Expression);糖基转移酶类(Glycosyltransferases);高通量筛选分析(High-Throughput Screening Assays);重组蛋白质类(Recombinant Proteins)

DOI 10.1371/journal.pone.0177591

PMID 28598995

发布时间 2019-02-08

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