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Heparinase enables reliable quantification of circulating tumor DNA from heparinized plasma samples by droplet digital PCR.

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第一作者： David,Sefrioui

第一单位： Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine and Digestive Oncology Unit, F 76000 Rouen, France.

作者： David,Sefrioui ^[1] ; Ludivine,Beaussire ^[2] ; Florian,Clatot ^[3] ; Julien,Delacour ^[2] ; Anne,Perdrix ^[4] ; Thierry,Frebourg ^[2] ; Pierre,Michel ^[1] ; Frédéric,Di Fiore ^[5] ; Nasrin,Sarafan-Vasseur ^[6]

作者单位： Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine and Digestive Oncology Unit, F 76000 Rouen, France. ^[1] Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine, F 76000 Rouen, France. ^[2] Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine and Department of Medical Oncology, Henri Becquerel Centre, F 76000 Rouen, France. ^[3] Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine and Department of Biopathology, Henri Becquerel Centre, F 76000 Rouen, France. ^[4] Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine, Digestive Oncology Unit and Department of Medical Oncology, Henri Becquerel Centre, F 76000 Rouen, France. ^[5] Normandie Univ, UNIROUEN, Inserm U1245, IRON group, Rouen University Hospital, Normandy Centre for Genomic and Personalized Medicine, F 76000 Rouen, France. Electronic address: Nasrin.Vasseur@univ-rouen.fr. ^[6]

关键词 Cell-free DNA Circulating tumor DNA Droplet digital PCR ESR1 Heparin Heparinase KRAS

医学主题词依地酸(Edetic Acid);肝素裂合酶(Heparin Lyase);人类(Humans);聚合酶链反应(Polymerase Chain Reaction);结果可重复性(Reproducibility of Results)

DOI 10.1016/j.cca.2017.07.015

PMID 28729136

发布时间 2018-04-23

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