A simple, cost-effective method for generating murine colonic 3D enteroids and 2D monolayers for studies of primary epithelial cell function.

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作者： Elizabeth H,Fernando ^[1] ; Michael,Dicay ^[1] ; Martin,Stahl ^[2] ; Marilyn H,Gordon ^[1] ; Andrew,Vegso ^[1] ; Cristiane,Baggio ^[1] ; Laurie,Alston ^[1] ; Fernando,Lopes ^[1] ; Kristi,Baker ^[3] ; Simon,Hirota ^[1] ; Derek M,McKay ^[1] ; Bruce,Vallance ^[2] ; Wallace K,MacNaughton ^[4]

作者单位： Department of Physiology and Pharmacology and Gastrointestinal Research Group, University of Calgary, Calgary, Alberta, Canada. ^[1] Department of Pediatrics, British Columbia Children's Hospital, University of British Columbia, Vancouver, British Columbia, Canada; and. ^[2] Department of Oncology, University of Alberta, Edmonton, Alberta, Canada. ^[3] Department of Physiology and Pharmacology and Gastrointestinal Research Group, University of Calgary, Calgary, Alberta, Canada; wmacnaug@ucalgary.ca. ^[4]

关键词 epithelial monolayers intestine mouse primary cell culture

主题词动物(Animals);细胞, 培养的(Cells, Cultured);结肠(Colon);上皮细胞(Epithelial Cells);肠黏膜(Intestinal Mucosa);小鼠(Mice);类器官(Organoids)

DOI 10.1152/ajpgi.00152.2017

PMID 28751424

发布时间 2017-11-28

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