The low molecular weight fraction of human serum albumin upregulates COX2, prostaglandin E2, and prostaglandin D2 under inflammatory conditions in osteoarthritic knee synovial fibroblasts.

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作者： Elizabeth D,Frederick ^[1] ; Melissa A,Hausburg ^[2] ; Gregory W,Thomas ^[1] ; Leonard T,Rael ^[2] ; Edward,Brody ^[1] ; David,Bar-Or ^[2]

作者单位： Swedish Medical Center, 501 E. Hampden Ave, Englewood, CO 80113, USA. ^[1] Ampio Pharmaceuticals Inc., 373 Inverness Pkwy, Englewood, CO 80112, USA. ^[2] SomaLogic Inc., 2945 Wilderness Place, Boulder, CO 80301, USA. ^[3] St. Anthony Hospital, Trauma Research Department, 11600 W. 2nd Pl, Lakewood, CO 80228, USA. ^[4] Medical Center of Plano, Trauma Research Department, 3901 W. 15th St., Plano, TX 75075, USA. ^[5] Rocky Vista University, 8401 S. Chambers Rd., Parker, CO 80134, USA. ^[6] Penrose Hospital, 2417 Cascade Ave, Colorado Springs, CO 80907, USA. ^[7]

关键词 15d-PGJ2, 15-deoxy-Δ12,14-prostaglandin J2 COX, cyclooxygenase Cyclooxygenase 2 DMEM/F12, Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 ELISA, enzyme-linked immunosorbent assay FBS, fetal bovine serum HSA, human serum albumin HSF-OAs, human synovial fibroblasts from patients with osteoarthritis Human serum albumin IL, interleukin IgG, immunoglobulin G Inflammation LMWF-5A, molecular weight fraction of human serum albumin under 5000?Da LOD, limit of detection NF-κB, nuclear factor-κB NSAIDs, non-steroidal anti-inflammatory drugs OA, osteoarthritis OAK, osteoarthritis of the knee Osteoarthritis PBMCs, peripheral blood mononuclear cells PG, prostaglandin PGD2, prostaglandin D2 PGE2, prostaglandin E2 PGH2, prostaglandin H2 PPARγ, peroxisome proliferator-activated receptor γ PVDF, polyvinylidene fluoride Prostaglandin SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis SEM, standard error of the mean Synovial fibroblast TNF, tumor necrosis factor hMSCs, human mesenchymal stem cells qPCR, quantitative real-time polymerase chain reaction ΔΔCT, comparative threshold cycle

DOI 10.1016/j.bbrep.2016.08.015

PMID 28955943

发布时间 2022-03-17

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Biochemistry and biophysics reports

2016年8卷

68-74页

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The low molecular weight fraction of human serum albumin upregulates COX2, prostaglandin E2, and prostaglandin D2 under inflammatory conditions in osteoarthritic knee synovial fibroblasts.

Biochemistry and biophysics reports

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Biochemistry and biophysics reports

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The low molecular weight fraction of human serum albumin upregulates COX2, prostaglandin E2, and prostaglandin D2 under inflammatory conditions in osteoarthritic knee synovial fibroblasts.

Biochemistry and biophysics reports

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Biochemistry and biophysics reports

相关期刊

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