换一批Effects of isoleucine 135 side chain length on the cofactor donor-acceptor distance within F<sub>420</sub>H<sub>2</sub>:NADP<sup>+</sup> oxidoreductase: A kinetic analysis.
第一作者：
Cuong Quang,Le
第一单位：
Department of Chemistry and Biochemistry, University of Texas at Arlington, TX 76019-0065, USA.
作者：
关键词
Dissociation constantsE. coli,, Escherichia coliF420 cofactorF420 cofactor, 7,8-didemethyl-8-hydroxy-5-deazariboflavin-5′-phosphoryllactyl(glutamyl)nglutamate, A. fulgidus, Archaeoglobus fulgidusF420H2: NADP+ oxidoreductaseFO, precursor of F420 cofactorFno, F420H2:NADP+, oxidoreductaseHalf-site reactivityI135, Isoleucine 135IPTG, isopropyl β-D-1-thiogalactopyranosideKd,, dissociation constantKm, Michaelis-Menten constantLB, Luria Bertani brothNADPNADP+, nicotinamide adenine dinucleotide phosphateNegative cooperativityPEI, PolyethyleneiminePre steady-state kineticsSteady-state kineticsk, rate constantkcat, catalytic rate constant (turnover number), kcat /Km, catalytic efficiencywtFno, wild-type Fno
DOI
10.1016/j.bbrep.2016.11.012
PMID
28955995
发布时间
2020-09-30
- 浏览12
Biochemistry and biophysics reports
2017年9卷
114-120页
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