Intrinsic thermodynamics of high affinity inhibitor binding to recombinant human carbonic anhydrase IV.

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作者： Aurelija,Mickevičiūtė ^[1] ; David D,Timm ^[1] ; Marius,Gedgaudas ^[1] ; Vaida,Linkuvienė ^[1] ; Zhiwei,Chen ^[2] ; Abdul,Waheed ^[2] ; Vilma,Michailovienė ^[1] ; Asta,Zubrienė ^[1] ; Alexey,Smirnov ^[1] ; Edita,Čapkauskaitė ^[1] ; Lina,Baranauskienė ^[1] ; Jelena,Jachno ^[1] ; Jurgita,Revuckienė ^[1] ; Elena,Manakova ^[3] ; Saulius,Gražulis ^[3] ; Jurgita,Matulienė ^[1] ; Enrico,Di Cera ^[2] ; William S,Sly ^[2] ; Daumantas,Matulis ^[4]

作者单位： Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, 10257, Vilnius, Lithuania. ^[1] Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO, 63104, USA. ^[2] Department of Protein-DNA Interactions, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, 10257, Vilnius, Lithuania. ^[3] Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, 10257, Vilnius, Lithuania. matulis@ibt.lt. ^[4]

关键词 Carbonic anhydrase Differential scanning fluorimetry Fluorescent thermal shift assay Intrinsic thermodynamics of protein–ligand binding Isothermal titration calorimetry X-ray crystallography