Isolation and characterization of ventricular-like cells derived from NKX2-5<sup>eGFP/w</sup> and MLC2v<sup>mCherry/w</sup> double knock-in human pluripotent stem cells.

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作者： Kaori,Yamauchi ^[1] ; Junjun,Li ^[2] ; Kumi,Morikawa ^[3] ; Li,Liu ^[2] ; Yasuaki,Shirayoshi ^[3] ; Norio,Nakatsuji ^[4] ; David A,Elliott ^[5] ; Ichiro,Hisatome ^[3] ; Hirofumi,Suemori ^[6]

作者单位： Laboratory of Embryonic Stem Cell Research, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. ^[1] Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Yoshida-ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan. ^[2] Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-machi, Yonago 683-8504, Japan. ^[3] Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Yoshida-ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan; Laboratory of Developmental Epigenome, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. ^[4] Murdoch Children's Research Institute, The RoyalChildren's Hospital, Parkville, Victoria 3052, Australia. ^[5] Laboratory of Embryonic Stem Cell Research, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. Electronic address: hsuemori@frontier.kyoto-u.ac.jp. ^[6]

关键词 Differentiation MLC2v NKX2-5 Ventricular cell hPSCs