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Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems.

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第一作者： Levi G,Lowder

第一单位： Department of Biology, East Carolina University, Greenville, NC 27858, USA.

作者： Levi G,Lowder ^[1] ; Jianping,Zhou ^[2] ; Yingxiao,Zhang ^[3] ; Aimee,Malzahn ^[3] ; Zhaohui,Zhong ^[2] ; Tzung-Fu,Hsieh ^[4] ; Daniel F,Voytas ^[5] ; Yong,Zhang ^[6] ; Yiping,Qi ^[7]

作者单位： Department of Biology, East Carolina University, Greenville, NC 27858, USA. ^[1] Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China. ^[2] Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA. ^[3] Department of Plant and Microbial Biology and Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA. ^[4] Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA. ^[5] Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China. Electronic address: zhangyong916@uestc.edu.cn. ^[6] Department of Biology, East Carolina University, Greenville, NC 27858, USA; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA. Electronic address: yiping@umd.edu. ^[7]

关键词 CRISPR MS2-VP64 TALE-VP64 gRNA2.0 multiplex transcriptional activation

医学主题词基因表达调控, 植物(Gene Expression Regulation, Plant);转录因子(Transcription Factors);转录激活(Transcriptional Activation)

DOI 10.1016/j.molp.2017.11.010

PMID 29197638

发布时间 2022-04-09

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