Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.

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作者： Taylor B,Cook ^[1] ; Jacqueline M,Rand ^[1] ; Wasti,Nurani ^[1] ; Dylan K,Courtney ^[1] ; Sophia A,Liu ^[1] ; Brian F,Pfleger ^[2]

作者单位： Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. ^[1] Waunakee High School, Waunakee, USA. ^[2] Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. pfleger@engr.wisc.edu. ^[3] Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, USA. pfleger@engr.wisc.edu. ^[4]

关键词 Cas9 recombineering Copy number LacI Promoter Pseudomonas putida Synthetic biology

主题词细菌蛋白质类(Bacterial Proteins);大肠杆菌(Escherichia coli);基因表达调控, 细菌(Gene Expression Regulation, Bacterial);质粒(Plasmids);启动区, 遗传(Promoter Regions, Genetic);恶臭假单胞菌(Pseudomonas putida);合成生物学(Synthetic Biology)

DOI 10.1007/s10295-017-2001-5

PMID 29299733

发布时间 2019-09-11

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