首页> Molecular cell> Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.

导出 OA

翻译打印收藏纠错

第一作者： Ryan,Marshall

第一单位： School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA.

作者： Ryan,Marshall ^[1] ; Colin S,Maxwell ^[2] ; Scott P,Collins ^[2] ; Thomas,Jacobsen ^[2] ; Michelle L,Luo ^[2] ; Matthew B,Begemann ^[3] ; Benjamin N,Gray ^[3] ; Emma,January ^[3] ; Anna,Singer ^[3] ; Yonghua,He ^[3] ; Chase L,Beisel ^[4] ; Vincent,Noireaux ^[5]

作者单位： School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA. ^[1] Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA. ^[2] Benson Hill Biosystems, St. Louis, MO 63132, USA. ^[3] Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: cbeisel@ncsu.edu. ^[4] School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: noireaux@umn.edu. ^[5]

关键词 Cas9 Cascade Cpf1 PAM TXTL prototyping synthetic biology

医学主题词无细胞系统(Cell-Free System);DNA, 细菌(DNA, Bacterial);内切核酸酶类(Endonucleases);大肠杆菌(Escherichia coli);遗传工程(Genetic Engineering);蛋白质生物合成(Protein Biosynthesis);转录, 遗传(Transcription, Genetic)

DOI 10.1016/j.molcel.2017.12.007

PMID 29304331

发布时间 2024-01-04

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交