An efficient screening method for purifying and crystallizing membrane proteins using modified clear-native PAGE.

导出 LinkOut

翻译打印收藏纠错

第一作者： Nanao,Suzuki

第一单位： Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan.

作者： Nanao,Suzuki ^[1] ; Yuuki,Takamuku ^[1] ; Tomohiro,Asakawa ^[2] ; Makoto,Inai ^[2] ; Tomoya,Hino ^[3] ; So,Iwata ^[4] ; Toshiyuki,Kan ^[2] ; Takeshi,Murata ^[5]

作者单位： Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan. ^[1] School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. ^[2] Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101, Koyama-cho Minami, Tottori 680-8552, Japan. ^[3] Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. ^[4] Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan; JST, PRESTO, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan; Molecular Chirality Research Center, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan. Electronic address: t.murata@faculty.chiba-u.jp. ^[5]

关键词 Clear-native PAGE Crystallization G protein-coupled receptor Membrane proteins Purification Stability

主题词结晶学, X线(Crystallography, X-Ray);人类(Humans);受体, 腺苷A2A(Receptor, Adenosine A2A);重组蛋白质类(Recombinant Proteins)

DOI 10.1016/j.ab.2018.02.007

PMID 29432751

发布时间 2019-03-06

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交