Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering.

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作者： Ikuko,Watakabe ^[1] ; Hisashi,Hashimoto ^[2] ; Yukiko,Kimura ^[1] ; Saori,Yokoi ^[3] ; Kiyoshi,Naruse ^[4] ; Shin-Ichi,Higashijima ^[5]

作者单位： 1National Institutes of Natural Sciences, Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, Higashiyama 5-1, Myodaiji, Okazaki, Aichi 444-8787 Japan. ^[1] 2Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601 Japan. ^[2] 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi 444-8787 Japan. ^[3] 3National Institutes of Natural Sciences, National Institute for Basic Biology, Okazaki, Aichi 444-8585 Japan. ^[4] 5Present address: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812 Japan. ^[5]

关键词 CRISPR/Cas9 Knock-in Medaka Transgenic

DOI 10.1186/s40851-017-0086-3

PMID 29445519

发布时间 2022-03-16

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Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering.

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Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering.

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