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Baculovirus-driven protein expression in insect cells: A benchmarking study.

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第一作者: Peggy,Stolt-Bergner
第一单位: Vienna Biocenter Core Facilities GmbH, Dr. Bohrgasse 3, 1030 Vienna, Austria.
作者单位: Vienna Biocenter Core Facilities GmbH, Dr. Bohrgasse 3, 1030 Vienna, Austria. [1] Max-Planck Institute of Biochemistry, Structural Cell Biology Department, Am Klopferspitz 18, 82152 Martinsried, Germany. [2] Lead Discovery Center GmbH, Otto-Hahn-Str. 15, 44227 Dortmund, Germany. [3] European Molecular Biology Laboratory (EMBL) Heidelberg, Protein Expression and Purification Core Facility, 69117 Heidelberg, Germany. [4] NKI Protein Facility, Division of Biochemistry, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. [5] Protein Expression Facility, The University of Queensland, Brisbane, Queensland 4072, Australia. [6] Protein Expression Purification and Characterization Facility, Max Planck Institute of Molecular Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, D-01307 Dresden, Germany. [7] Helmholtz Center Munich, Protein Expression and Purification Facility, Institute of Structural Biology, Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany. [8] Structural Biology Laboratory, Elettra-Sincrotrone Trieste S.C.p.A., Trieste 34149, Italy. [9] Helmholtz Centre for Infection Research, Department Structure and Function of Proteins, Inhoffenstrasse 7, 38124 Braunschweig, Germany. [10] Lund University, Lund Protein Production Platform (LP3), Sölvegatan 35, SE-223 62 Lund, Sweden. [11] Novartis Institutes for BioMedical Research, Emeryville, CA, USA. [12] Max Planck Institute of Molecular Physiology, Dortmund Protein Facility, Otto-Hahn-Str. 11, 44227 Dortmund, Germany. [13] Max-Planck Institute of Biochemistry, Biochemistry Core Facility, Am Klopferspitz 18, 82152 Martinsried, Germany. [14] Coriolis Pharma, Biopharmaceutical Research and Development Service, Am Klopferspitz 19, 82152 Martinsried, Germany. [15] Protein Expression Facility, The University of Queensland, Brisbane, Queensland 4072, Australia. Electronic address: l.lua@uq.edu.au. [16] Max-Planck Institute of Biochemistry, Biochemistry Core Facility, Am Klopferspitz 18, 82152 Martinsried, Germany. Electronic address: suppmann@biochem.mpg.de. [17]
DOI 10.1016/j.jsb.2018.03.004
PMID 29545204
发布时间 2019-10-07
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Journal of structural biology

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