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关键词
Acidic PNGaseFNG: free N-glycanFuc: L-fucoseGal: D-galactoseGlcNAc: N-acetyl-D-glucosamineHPLC: high-performance liquid chromatographyMan: D-mannoseNeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcNeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAcNeuNAc: N-acetylneuraminic acidPA-: pyridylaminoPNGase-A: aPNGase from almond seedPNGase-Le: aPNGase from tomato (Solanum lycopersium L.)PNGase: peptide:N-glycanasePTC: plant complex typeRCA120: Ricinus communis agglutinin (120?kDa)RP-HPLC: reversed-phase HPLCSF-HPLC: size-fractionation HPLCXyl: D-xyloseaffinity chromatographyenzyme assayfree N-glycanstransgenic plant
主题词
氨基酸序列(Amino Acid Sequence);拟南芥属(Arabidopsis);色谱法, 液相(Chromatography, Liquid);荧光染料(Fluorescent Dyes);糖肽类(Glycopeptides);肽-N4-(N-乙酰-β-葡糖胺)天冬酰胺酰胺酶(Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase);植物提取物(Plant Extracts);植物叶(Plant Leaves);植物, 基因修饰(Plants, Genetically Modified);底物特异性(Substrate Specificity)
DOI
10.1080/09168451.2018.1459464
PMID
29658432
发布时间
2022-12-07
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