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A RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum.

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第一作者： Bo,Wang

第一单位： CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.;University of Chinese Academy of Sciences, Beijing, 100049, China.

作者： Bo,Wang ^[1] ; Qitiao,Hu ^[1] ; Yu,Zhang ^[1] ; Ruilin,Shi ^[2] ; Xin,Chai ^[2] ; Zhe,Liu ^[1] ; Xiuling,Shang ^[3] ; Yun,Zhang ^[4] ; Tingyi,Wen ^[5]

作者单位： CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.;University of Chinese Academy of Sciences, Beijing, 100049, China. ^[1] Beijing Zhongke Eppen Biotech Co., Ltd, Beijing, 100085, China. ^[2] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. ^[3] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. zhangyun@im.ac.cn. ^[4] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. wenty@im.ac.cn.;Beijing Zhongke Eppen Biotech Co., Ltd, Beijing, 100085, China. wenty@im.ac.cn.;Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China. wenty@im.ac.cn. ^[5]

关键词 1,2-Propanediol CRISPR–Cas9 Corynebacterium glutamicum Genome editing Transformation efficiency recET-encoded recombinase

医学主题词谷氨酸棒状杆菌(Corynebacterium glutamicum)

DOI 10.1186/s12934-018-0910-2

PMID 29685154

发布时间 2024-01-04

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