Towards the construction of high-quality mutagenesis libraries.

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作者： Heng,Li ^[1] ; Jing,Li ^[2] ; Ruinan,Jin ^[1] ; Wei,Chen ^[2] ; Chaoning,Liang ^[1] ; Jieyuan,Wu ^[1] ; Jian-Ming,Jin ^[1] ; Shuang-Yan,Tang ^[1]

作者单位： CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, Beijing, 100101, China. ^[1] University of Chinese Academy of Sciences, Beijing, 100049, China. ^[2] Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, 100048, China. ^[3] CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, Beijing, 100101, China. tangsy@im.ac.cn. ^[4]

关键词 Directed evolution Multiple-plasmid transformant Mutagenesis library Transformation Transformation efficiency

主题词定向分子进化(Directed Molecular Evolution);大肠杆菌(Escherichia coli);基因文库(Gene Library);质粒(Plasmids);转化, 细菌(Transformation, Bacterial)

DOI 10.1007/s10529-018-2559-7

PMID 29700725

发布时间 2018-09-27

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准确一般字义偏差语意偏离译文作用小提交