Randomized DNA libraries construction tool: a new 3-bp 'frequent cutter' TthHB27I/sinefungin endonuclease with chemically-induced specificity.

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作者： Daria,Krefft ^[1] ; Aliaksei,Papkov ^[1] ; Maciej,Prusinowski ^[1] ; Agnieszka,Zylicz-Stachula ^[1] ; Piotr M,Skowron ^[2]

作者单位： Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland. ^[1] Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland. piotr.skowron@ug.edu.pl. ^[2]

关键词 DNA fragmentation Genomic libraries Restriction endonuclease-methyltransferase Specificity relaxation Star activity Thermophile

主题词克隆, 分子(Cloning, Molecular);DNA裂解(DNA Cleavage);脱氧核糖核酸酶Ⅰ(Deoxyribonuclease I);基因文库(Gene Library);基因组学(Genomics);底物特异性(Substrate Specificity)

DOI 10.1186/s12864-018-4748-0

PMID 29751745

发布时间 2018-12-17

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