Enhanced catalytic activities and modified substrate preferences for taxoid 10β-O-acetyl transferase mutants by engineering catalytic histidine residues.

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第一作者： Lin-Feng,You

第一单位： College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, Guangdong, China.;Chongqing Key Laboratory of Catalysis and Functional Organic Molecule, Chongqing Technology and Business University, Chongqing, 400067, China.

作者： Lin-Feng,You ^[1] ; Jia-Jun,Huang ^[2] ; Tao,Wei ^[3] ; Shu-Ling,Lin ^[2] ; Bing-Hua,Jiang ^[4] ; Li-Qiong,Guo ^[5] ; Jun-Fang,Lin ^[6]

作者单位： College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, Guangdong, China.;Chongqing Key Laboratory of Catalysis and Functional Organic Molecule, Chongqing Technology and Business University, Chongqing, 400067, China. ^[1] College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, Guangdong, China. ^[2] College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, Guangdong, China.;Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, 510640, Guangdong, China. ^[3] Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, 19107, USA. ^[4] College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, Guangdong, China. guolq@scau.edu.cn.;Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, 510640, Guangdong, China. guolq@scau.edu.cn. ^[5] College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, Guangdong, China. linjf@scau.edu.cn.;Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, 510640, Guangdong, China. linjf@scau.edu.cn. ^[6]

关键词 Acetylation Baccatin III Site-directed mutagenesis Taxoid 10β-O-acetyl transferase Unnatural acetyl donor