Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion.

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作者： Jing,Luo ^[1] ; Liaoxun,Lu ^[2] ; Yanrong,Gu ^[1] ; Rong,Huang ^[1] ; Lin,Gui ^[1] ; Saichao,Li ^[1] ; Xinhui,Qi ^[1] ; Wenping,Zheng ^[1] ; Tianzhu,Chao ^[3] ; Qianqian,Zheng ^[1] ; Yinming,Liang ^[4] ; Lichen,Zhang ^[5]

作者单位： Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, Henan Province 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan, China. ^[1] Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, Henan Province 453003, China; Laboratory of Mouse Genetics, Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Henan Province, 453003, China. ^[2] Laboratory of Mouse Genetics, Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Henan Province, 453003, China. ^[3] Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, Henan Province 453003, China; Laboratory of Mouse Genetics, Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Henan Province, 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address: yinming.liang@foxmail.com. ^[4] Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, Henan Province 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address: zhanglichen@xxmu.edu.cn. ^[5]

关键词 CRISPR/Cas9 Fluorescent reporter Large DNA fragment deletion Single cell sorting Speed genome editing