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Perspective: next generation isotope-aided methods for protein NMR spectroscopy.

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第一作者： Masatsune,Kainosho

第一单位： Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo, 192-0397, Japan. kainosho@tmu.ac.jp.;Structural Biology Research Center, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan. kainosho@tmu.ac.jp.

作者： Masatsune,Kainosho ^[1] ; Yohei,Miyanoiri ^[2] ; Tsutomu,Terauchi ^[3] ; Mitsuhiro,Takeda ^[4]

作者单位： Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo, 192-0397, Japan. kainosho@tmu.ac.jp.;Structural Biology Research Center, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan. kainosho@tmu.ac.jp. ^[1] Structural Biology Research Center, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.;Research Center for State-of-the-Art Functional Protein Analysis, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. ^[2] SI Innovation Center, Taiyo Nippon Sanso Corp., 2008-2 Wada, Tama-city, Tokyo, 206-0001, Japan. ^[3] Department of Structural BioImaging, Faculty of Life Sciences, Kumamoto University, 5-1, Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan. ^[4]

关键词 1H-direct observation at ultrahigh-field Isotope-aided NMR method for larger proteins SAIL aliphatic 13CH TROSY SAIL aromatic 13CH TROSY Stereo-array isotope labeling (SAIL)TROSY by isotope labeling

医学主题词氨基酸类(Amino Acids);氨基酸类, 芳香(Amino Acids, Aromatic);碳同位素(Carbon Isotopes);同位素标记(Isotope Labeling);核磁共振, 生物分子(Nuclear Magnetic Resonance, Biomolecular);蛋白质构象(Protein Conformation)

DOI 10.1007/s10858-018-0198-x

PMID 29934841

发布时间 2019-04-15

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