Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset.

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作者： Charlotte M,Miton ^[1] ; Stefanie,Jonas ^[1] ; Gerhard,Fischer ^[1] ; Fernanda,Duarte ^[2] ; Mark F,Mohamed ^[1] ; Bert,van Loo ^[1] ; Bálint,Kintses ^[1] ; Shina C L,Kamerlin ^[2] ; Nobuhiko,Tokuriki ^[1] ; Marko,Hyvönen ^[3] ; Florian,Hollfelder ^[1]

作者单位： Department of Biochemistry, University of Cambridge, CB2 1GA Cambridge, United Kingdom. ^[1] Department of Chemistry, Biomedicinskt Centrum (BMC), Uppsala University, 751 23 Uppsala, Sweden. ^[2] Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. ^[3] Department of Biochemistry, University of Cambridge, CB2 1GA Cambridge, United Kingdom; fh111@cam.ac.uk. ^[4]

关键词 catalytic promiscuity directed evolution enzyme–substrate complementarity linear free-energy relationship phosphate transfer

主题词芳基硫酸酯酶类(Arylsulfatases);催化作用(Catalysis);催化域(Catalytic Domain);定向分子进化(Directed Molecular Evolution);水解作用(Hydrolysis);有机磷化合物(Organophosphorus Compounds);蛋白质构象(Protein Conformation)

DOI 10.1073/pnas.1607817115

PMID 30012610

发布时间 2019-01-31

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