High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue.

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第一作者： Liza,Konnikova

第一单位： Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA.;Department of Pediatrics and Newborn Medicine, Brigham and Women's Hospital, Boston, MA, 02115, USA.;Harvard Medical School, Boston, MA, 02115, USA.

作者： Liza,Konnikova ^[1] ; Gilles,Boschetti ^[2] ; Adeeb,Rahman ^[2] ; Vanessa,Mitsialis ^[3] ; James,Lord ^[4] ; Camilla,Richmond ^[5] ; Vesselin T,Tomov ^[6] ; Will,Gordon ^[7] ; Scott,Jelinsky ^[7] ; James,Canavan ^[8] ; Andrew,Liss ^[9] ; Sarah,Wall ^[10] ; Michael,Field ^[10] ; Fanny,Zhou ^[9] ; Jeffery D,Goldsmith ^[11] ; Meenakshi,Bewtra ^[6] ; David T,Breault ^[12] ; Miriam,Merad ^[2] ; Scott B,Snapper ^[13]

作者单位： Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA.;Department of Pediatrics and Newborn Medicine, Brigham and Women's Hospital, Boston, MA, 02115, USA.;Harvard Medical School, Boston, MA, 02115, USA. ^[1] Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. ^[2] Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, 02115, USA. ^[3] Benaroya Research Institute, Seattle, WA, 98101, USA. ^[4] Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA.;Harvard Medical School, Boston, MA, 02115, USA. ^[5] Department of Medicine, Division of Gastroenterology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA. ^[6] Pfizer, Cambridge, MA, 02139, USA. ^[7] Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA.;Harvard Medical School, Boston, MA, 02115, USA.;Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, 02115, USA. ^[8] Department of Pathology, Boston Children's Hospital, Boston, MA, 02115, USA. ^[9] Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA. ^[10] Harvard Medical School, Boston, MA, 02115, USA.;Department of Pathology, Boston Children's Hospital, Boston, MA, 02115, USA. ^[11] Harvard Medical School, Boston, MA, 02115, USA.;Division of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA.;Harvard Stem Cell Institute, Cambridge, MA, 02138, USA. ^[12] Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA. scott.snapper@childrens.harvard.edu.;Harvard Medical School, Boston, MA, 02115, USA. scott.snapper@childrens.harvard.edu.;Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, 02115, USA. scott.snapper@childrens.harvard.edu. ^[13]