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首页> Ecotoxicology and environmental safety> Secretory expression, immunoaffinity purification and metal-binding ability of recombinant metallothionein (ShMT) from freshwater crab Sinopotamon henanense.

Secretory expression, immunoaffinity purification and metal-binding ability of recombinant metallothionein (ShMT) from freshwater crab Sinopotamon henanense.

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作者： Yongji,He ^[1] ; Lan,Wang ^[2] ; Wenli,Ma ^[2] ; Xiaoxia,Lu ^[3] ; Yunlong,Li ^[4] ; Jinping,Liu ^[2]

作者单位： Institute of Agro-Products Processing Science and Technology, Shanxi Academy of Agricultural Sciences, 79 Longcheng Street, Taiyuan 030031, Shanxi Province, PR China; School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, Shanxi Province, PR China. Electronic address: heyongji_919@163.com. ^[1] School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, Shanxi Province, PR China. ^[2] Biology Institute of Shanxi, 50 Shifan Street, Taiyuan 030006, Shanxi Province, PR China. ^[3] Institute of Agro-Products Processing Science and Technology, Shanxi Academy of Agricultural Sciences, 79 Longcheng Street, Taiyuan 030031, Shanxi Province, PR China. ^[4]

关键词 Immunoaffinity purification Metal-binding ability Metallothionein Polyol-responsive Secretory expression Sinopotamon henanense

主题词氨基酸序列(Amino Acid Sequence);动物(Animals);短尾派(Brachyura);中国(China);色谱法, 亲和(Chromatography, Affinity);酶联免疫吸附测定(Enzyme-Linked Immunosorbent Assay);大肠杆菌(Escherichia coli);基因表达调控(Gene Expression Regulation);金属硫蛋白(Metallothionein);金属, 重(Metals, Heavy);蛋白质结合(Protein Binding);蛋白质亚型(Protein Isoforms);重组蛋白质类(Recombinant Proteins)

DOI 10.1016/j.ecoenv.2018.11.065

PMID 30472469

发布时间 2019-01-31

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Ecotoxicology and environmental safety

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