Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA.

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作者： Meiling,Gao ^[1] ; Maurizio,Callari ^[1] ; Emma,Beddowes ^[1] ; Stephen-John,Sammut ^[2] ; Marta,Grzelak ^[1] ; Heather,Biggs ^[1] ; Linda,Jones ^[2] ; Abdelhamid,Boumertit ^[2] ; Sabine C,Linn ^[2] ; Javier,Cortes ^[3] ; Mafalda,Oliveira ^[4] ; Richard,Baird ^[5] ; Suet-Feung,Chin ^[5] ; Carlos,Caldas ^[2]

作者单位： Department of Oncology and Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge, CB2 0RE, UK. ^[1] Breast Cancer Programme, Cancer Research UK Cambridge Cancer Centre, Cambridge University Hospitals NHS Foundation Trust, Cambridge, CB2 2QQ, UK. ^[2] Netherland Cancer Institute, 1006 BE, Amsterdam, Netherlands. ^[3] Ramon y Cajal University Hospital, 28034, Madrid, Spain. ^[4] Vall d'Hebron Institute of Oncology, 08035, Barcelona, Spain. ^[5] Department of Oncology and Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge, CB2 0RE, UK. Suet-Feung.Chin@cruk.cam.ac.uk. ^[6] Department of Oncology and Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge, CB2 0RE, UK. carlos.caldas@cruk.cam.ac.uk. ^[7] Breast Cancer Programme, Cancer Research UK Cambridge Cancer Centre, Cambridge University Hospitals NHS Foundation Trust, Cambridge, CB2 2QQ, UK. carlos.caldas@cruk.cam.ac.uk. ^[8]

关键词 Cancer Computational pipeline Deep sequencing Heterogeneous Liquid biopsy Multiplexing Mutation NG-TAS ctDNA