Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response.

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作者： Masoud,Nasri ^[1] ; Perihan,Mir ^[1] ; Benjamin,Dannenmann ^[2] ; Diana,Amend ^[1] ; Tessa,Skroblyn ^[1] ; Yun,Xu ^[1] ; Klaus,Schulze-Osthoff ^[3] ; Maksim,Klimiankou ^[1] ; Karl,Welte ^[2] ; Julia,Skokowa ^[4]

作者单位： Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany. ^[1] German Cancer Research Center, Heidelberg, Germany. ^[2] Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany. ^[3] Interfaculty Institute of Biochemistry, Tübingen University, Tübingen, Germany; and. ^[4] University Children's Hospital Tübingen, Tübingen, Germany. ^[5]

主题词抗原, 分化(Antigens, Differentiation);细胞系(Cell Line);DNA损伤(DNA Damage);基因敲除技术(Gene Knockout Techniques);基因打靶(Gene Targeting);造血干细胞(Hematopoietic Stem Cells);人类(Humans);诱导多能干细胞(Induced Pluripotent Stem Cells);大分子物质(Macromolecular Substances);蛋白质结合(Protein Binding);核糖核蛋白类(Ribonucleoproteins);应激, 生理学(Stress, Physiological)

DOI 10.1182/bloodadvances.2017015511

PMID 30622144

发布时间 2024-01-04

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Blood advances

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Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response.

Blood advances

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Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response.

Blood advances

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Blood advances

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