Molecular Profiling of RNA Tumors Using High-Throughput RNA Sequencing: From Raw Data to Systems Level Analyses.

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作者： Willian A,da Silveira ^[1] ; E Starr,Hazard ^[2] ; Dongjun,Chung ^[1] ; Gary,Hardiman ^[3]

作者单位： MUSC Bioinformatics, Center for Genomics Medicine, Medical University of South Carolina (MUSC), Charleston, SC, USA. ^[1] Institute for Global Food Security, Queens University Belfast, Belfast, UK. ^[2] Library Science and Informatics, Medical University of South Carolina (MUSC), Charleston, SC, USA. ^[3] Department of Public Health Sciences, Medical University of South Carolina (MUSC), Charleston, SC, USA. ^[4] MUSC Bioinformatics, Center for Genomics Medicine, Medical University of South Carolina (MUSC), Charleston, SC, USA. G.Hardiman@qub.ac.uk. ^[5] Department of Public Health Sciences, Medical University of South Carolina (MUSC), Charleston, SC, USA. G.Hardiman@qub.ac.uk. ^[6] Department of Medicine, Medical University of South Carolina (MUSC), Charleston, SC, USA. G.Hardiman@qub.ac.uk. ^[7] School of Biological Sciences & Institute for Global Food Security, Queens University Belfast, Stranmillis Road, Belfast, BT9 5AG, UK. G.Hardiman@qub.ac.uk. ^[8]

关键词 DESeq 2 FASTQ Gene Set Enrichment Analysis/GSEA HTSeq High-throughput sequencing (HTS)Limma/Voom RNAsequencing (RNAseq)TOPPGENE Tuxedo pipeline