Cell-Free Protein Synthesis Using S30 Extracts from <i>Escherichia coli</i> RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins.

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作者： Jiro,Adachi ^[1] ; Kazushige,Katsura ^[2] ; Eiko,Seki ^[3] ; Chie,Takemoto ^[4] ; Mikako,Shirouzu ^[5] ; Takaho,Terada ^[6] ; Takahito,Mukai ^[7] ; Kensaku,Sakamoto ^[8] ; Shigeyuki,Yokoyama ^[9]

作者单位： RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. jiro.adachi@riken.jp. ^[1] Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystems Dynamics Research, Osaka 565-0874, Japan. jiro.adachi@riken.jp. ^[2] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. kazushige.katsura@riken.jp. ^[3] Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama 230-0045, Japan. kazushige.katsura@riken.jp. ^[4] Laboratory for Protein Functional and Structural Biology, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, Japan. kazushige.katsura@riken.jp. ^[5] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. eiko.seki@riken.jp. ^[6] RIKEN Structural Biology Laboratory, Yokohama 230-0045, Japan. eiko.seki@riken.jp. ^[7] RIKEN Cluster for Science, Technology and Innovation Hub, Yokohama 230-0045, Japan. eiko.seki@riken.jp. ^[8] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. chie.takemoto@riken.jp. ^[9] Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama 230-0045, Japan. chie.takemoto@riken.jp. ^[10] Laboratory for Protein Functional and Structural Biology, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, Japan. chie.takemoto@riken.jp. ^[11] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. mikako.shirouzu@riken.jp. ^[12] Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama 230-0045, Japan. mikako.shirouzu@riken.jp. ^[13] Laboratory for Protein Functional and Structural Biology, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, Japan. mikako.shirouzu@riken.jp. ^[14] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. tera@riken.jp. ^[15] RIKEN Structural Biology Laboratory, Yokohama 230-0045, Japan. tera@riken.jp. ^[16] RIKEN Cluster for Science, Technology and Innovation Hub, Yokohama 230-0045, Japan. tera@riken.jp. ^[17] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. takahito.mukai@rikkyo.ac.jp. ^[18] Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama 230-0045, Japan. takahito.mukai@rikkyo.ac.jp. ^[19] Department of Life Science, College of Science, Rikkyo University, Tokyo 171-8501, Japan. takahito.mukai@rikkyo.ac.jp. ^[20] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. kensaku.sakamoto@riken.jp. ^[21] Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama 230-0045, Japan. kensaku.sakamoto@riken.jp. ^[22] Laboratory for Nonnatural Amino Acid Technology, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, Japan. kensaku.sakamoto@riken.jp. ^[23] RIKEN Systems and Structural Biology Center, Yokohama 230-0045, Japan. yokoyama@riken.jp. ^[24] RIKEN Structural Biology Laboratory, Yokohama 230-0045, Japan. yokoyama@riken.jp. ^[25] RIKEN Cluster for Science, Technology and Innovation Hub, Yokohama 230-0045, Japan. yokoyama@riken.jp. ^[26]

关键词 3-iodo-l-tyrosine RF-1 RFzero-iy cell-free protein synthesis non-natural amino acid release factor 1